cryostor® cs10 cell preservation media  (BioLife Solutions)

Journal: Arthritis Research & Therapy

Article Title: Single-cell transcriptome conservation in a comparative analysis of fresh and cryopreserved human skin tissue: pilot in localized scleroderma

doi: 10.1186/s13075-020-02343-4

Figure Lengend Snippet: Total number of cells recovered and cell type recovery overall is comparable between preservation methods in frozen media (CryoStor® CS10) compared to fresh media (RPMI) in the 18 cell clusters within the 9 cell groupings

Article Snippet: These two biopsies were allocated for scRNAseq; one whole biopsy was placed in Roswell Park Memorial Institute (RPMI) 1640 Medium (Gibco®, Gaithersburg, MD) and put on ice while the other whole biopsy was placed in chilled CryoStor® CS10 cell preservation media (BioLife Solutions®, Bothell, WA), incubated at 4 °C for 10 min then put on dry ice per manufacturer’s instructions.

Techniques: Preserving

Journal: Arthritis Research & Therapy

Article Title: Single-cell transcriptome conservation in a comparative analysis of fresh and cryopreserved human skin tissue: pilot in localized scleroderma

doi: 10.1186/s13075-020-02343-4

Figure Lengend Snippet: Top 10 differentially expressed genes comparing paired frozen media (CryoStor® CS10) compared to fresh media (RPMI) skin samples. Full 123 DEGs are in Supplement

Article Snippet: These two biopsies were allocated for scRNAseq; one whole biopsy was placed in Roswell Park Memorial Institute (RPMI) 1640 Medium (Gibco®, Gaithersburg, MD) and put on ice while the other whole biopsy was placed in chilled CryoStor® CS10 cell preservation media (BioLife Solutions®, Bothell, WA), incubated at 4 °C for 10 min then put on dry ice per manufacturer’s instructions.

Techniques:

Journal: Arthritis Research & Therapy

Article Title: Single-cell transcriptome conservation in a comparative analysis of fresh and cryopreserved human skin tissue: pilot in localized scleroderma

doi: 10.1186/s13075-020-02343-4

Figure Lengend Snippet: Single-cell sequencing performance metrics are equivalent between skin specimens preserved in frozen media (CryoStor® CS10) compared to fresh media (RPMI)

Article Snippet: These two biopsies were allocated for scRNAseq; one whole biopsy was placed in Roswell Park Memorial Institute (RPMI) 1640 Medium (Gibco®, Gaithersburg, MD) and put on ice while the other whole biopsy was placed in chilled CryoStor® CS10 cell preservation media (BioLife Solutions®, Bothell, WA), incubated at 4 °C for 10 min then put on dry ice per manufacturer’s instructions.

Techniques: Sequencing, Preserving

Journal: Arthritis Research & Therapy

Article Title: Single-cell transcriptome conservation in a comparative analysis of fresh and cryopreserved human skin tissue: pilot in localized scleroderma

doi: 10.1186/s13075-020-02343-4

Figure Lengend Snippet: Transcriptomic expression of genes within cell types was similar between preservation methods in frozen media (CryoStor® CS10) compared to fresh media (RPMI). Strong spearman’s correlation coefficients were demonstrated among all cell types

Article Snippet: These two biopsies were allocated for scRNAseq; one whole biopsy was placed in Roswell Park Memorial Institute (RPMI) 1640 Medium (Gibco®, Gaithersburg, MD) and put on ice while the other whole biopsy was placed in chilled CryoStor® CS10 cell preservation media (BioLife Solutions®, Bothell, WA), incubated at 4 °C for 10 min then put on dry ice per manufacturer’s instructions.

Techniques: Expressing, Preserving